Transcriptome analysis shows that alkalinity affects metabolism in the roots of Mesembryanthemum crystallinum

Mesembryanthemum crystallinum is a model halophyte that switches from C3 photosynthesis to Crassulacean acid metabolism (CAM) upon extreme abiotic stresses. This study aimed investigate alkalinity-induced root transcriptome profiling in M. crystallinum. seedlings were treated with 50 mM sodium bicarbonate (NaHCO3; pH 7.5) and 90 NaHCO3 (pH 9.5) for 7 d, respectively. Alkalinity-induced differentially expressed genes (DEGs) identified annotated. Functional enrichment analysis was performed DEGs. The expression of related response stress CAM analyzed compared. Comparing control, treatments induced 4 027 25 403 DEGs roots, Among these DEGs, 832 131 consistently upregulated downregulated by both stresses, These associated multiple biological processes Alkaline encoding heat shock proteins ethylene-related genes, but glutathione S-transferases. Also, encode phosphoenolpyruvate carboxylases, carboxylase kinase 1, malate dehydrogenases accumulation alkalinity. indicated alkaline affected the responses, metabolism, roots